Partial purification of crude stem bromelain improves it sensitivity as a protease inhibitive assay for heavy metals
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چکیده
The protease inhibitive assay for heavy metals is a novel and robust detection method suitable for preliminary screening method for detecting heavy metals in agriculture and environment samples. Stem bromelain has been previously showed to be sensitive towards mercury and copper. However, commercial preparation is in crude form and contains possible interfering entities. Using ion exchange and gel filtration chromatography, a 5.6 fold purification was achieved. The partially purified fraction showed significantly lower IC50s (p<0.05) for both copper and mercury than the crude fraction demonstrating that partial purification improves the sensitivity of the bromelain inhibitive assay for heavy metals.
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